Table 1 Gene specific primer sequences used for qPCR
Pathway | Official symbol | Gene ID | Forward primer | Reverse primer | Annealing temp (°C) | Amplicon length (bp) | Target RefSeq |
|---|---|---|---|---|---|---|---|
Deiondination | DIO1 | 414380 | 5′-ACTTCATGCAAGGCAACAGG-3′ | 5′-GGTCCTGGAGATTCTGGTGA-3′ | 61 | 213 | NM_001001627.1 |
DIO2 | 414379 | 5′-CTCGGTCATTCTCCTCAAGC-3′ | 5′-TCACCTGTTTGTAGGCATCG-3′ | 61 | 140 | NM_001001626.2 | |
DIO3 | 414378 | 5′-CCTATCTGCGTGTCTGACGA-3′ | 5′-GCCTGCTTGAAGAAATCCAG-3′ | 61 | 92 | NM_001001625.2 | |
Sulfation | SULT1A3 | 396640 | 5′-GGTGTCCCACAGGTTTTGAG-3′ | 5′-CGACGTAGACCACCTTGACC-3′ | 61 | 123 | NM_213765 |
SULT1B1 | 100624541 | 5′-TGGCTGGAAATGTGGCTTAT-3′ | 5′-TCACCAGAGGGTTGTCCTTC-3′ | 61 | 228 | XM_005656511 | |
SULT1C2 | 100623541 | 5′-GAAACCTCAGTCAGCGGAAA-3′ | 5′-TTCGGGTTCCTCTTGATGTC-3′ | 61 | 125 | XM_013995894.2 | |
SULT1E1 | 397052 | 5′-GGGAGGAATTTTGGGTGACTA-3′ | 5′-GCCAGATTTGGGATAGGTGA-3′ | 61 | 204 | NM_213992.1 | |
SULT2A1 | 641359 | 5′-CCAAGGAAATGTGCCCTATG-3′ | 5′-GTCCCGTTTCAGCTCTTCAT-3′ | 61 | 109 | XM_005664644 | |
STS | 448816 | 5′-AGACCCTCAGGACTCCCAAT-3′ | 5′-AAACTCCCACCTGGTTCTGA-3′ | 61 | 162 | XM_021080518 | |
Transportation | SLC16A2 | 100513513 | 5′-AGTGGAGTTCCAAGCAGCAT-3′ | 5′-AGCCCAAACGATCAGTGAAT-3′ | 61 | 95 | XM_021080455.1 |
SLC16A10 | 100513770 | 5′-CACCCATTGCAGGGTTACTC-3′ | 5′-TATGGAGCCAAGGGATGAAA-3′ | 61 | 117 | XM_021091212.1 |