Table 1 Sequences of primers used in this study
Target gene | Primer | Sequence (5′–3′) | Accession number | Amplicon size (bp) | Gene region |
|---|---|---|---|---|---|
16S rRNA | 16S rRNA-F | AGTTGTTCTCAGTTCGGATT | NZ-CP031196.1 | 228 | 786,530–786,739 |
16S rRNA-R | ATACGGCTACCTTGTTACG | ||||
WalK | WalK-F | TTGATTACCGTGATACTTGG | NZ-CP031196.1 | 195 | 28,795–28,972 |
WalK-R | ATTCGCTTGTGCTTCTTG | ||||
WalR | WalR-F | GTATGGAAGTATGTCGTGAAG | NZ-CP031196.1 | 197 | 27,683–27,861 |
WalR-R | TTGTGCTGGTTGTGAGTAA | ||||
yycI | yycI-F | AGAAGGTGCGAATAACGATA | NZ-CP031196.1 | 127 | 31,912–32,019 |
yycI-R | AACGGTATAGTAGCCAAGTC | ||||
yycH | yycH-F | GCCATTAGCAACCTACTTAG | NZ-CP031196.1 | 262 | 30,421–30,663 |
yycH-R | TGTCGCTCTATCAATCGTAT |