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Fig. 5 | Veterinary Research

Fig. 5

From: A porcine reproductive and respiratory syndrome virus (PRRSV)-specific IgM as a novel adjuvant for an inactivated PRRSV vaccine improves protection efficiency and enhances cell-mediated immunity against heterologous PRRSV challenge

Fig. 5

Evaluation of PRRSV RNA and gene expression alterations in alveolar macrophages from surviving piglets in different groups. A. RNA from PAMs collected from necropsied piglets at 21 dpi was harvested using TRIzol reagent and further subjected to PRRSV RNA detection using IDEXX RealPCR PRRSV-2 RNA Mix. The Ct value of each sample is presented for comparison and was based on the manufacturer cut-off Ct value of 38. B. RNA from PAMs was harvested using TRIzol reagent for RNA extraction and reverse transcription. The expression of macrophage activation-related genes (M1 or M2) was examined by qPCR for the indicated genes. All experiments were repeated at least three times. Data are expressed as the mean ± SD.

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Veterinary Research

ISSN: 1297-9716