Expression level and subcellular localization of SaoA or Eno in rSC0016(pS-SE) with the double expression cassette. Subcellular fractions of SaoA and Eno in rSC0016(pS-SaoA) (A), rSC0016(pS-Eno) (B) and rSC0016(pS-SE) (A, B) from cells grown in NB detected by western blot. Representative images in A or B are from one of the three representative experiments. Densitometry was analyzed with the Image J software (C, D). ns: not significant.