Figure 6

Effects of rHcDR on Th9 cells and IL-9 expression in vitro. A Agarose gel electrophoresis of the HcDR gene. Lane 1: amplification products of the HcDR gene. Lane M: DNA molecular weight marker. B Purification of rHcDR. Lane 2: before purification. Lane 3: purified rHcDR. Lane M: standard protein molecular marker. C Western blot. Lane 4: rHcDR recognized by serum from an H. contortus-infected goat. Lane 5: No recognition by normal serum. Lane M: standard protein molecular marker. D The effects of rHcDR on Th9-cell proliferation. Goat PBMCs were treated with different concentrations of rHcDR (0, 5, 10, 20, 40 and 60 μg/mL). Th9 cells were detected by flow cytometry using staining with antibodies specific for typical intracellular cytokines (IL-9 and IL-10). E Proportions of Th9 cells observed with different concentrations of rHcDR (0, 5, 10, 20, 40 and 60 μg/mL). Data are presented as the mean ± SD and are representative of triplicate experiments (*p < 0.05, ****p < 0.0001). F Fold change in relative IL-9 mRNA expression. Goat PBMCs were stimulated with different concentrations of rHcDR. The significance level was set at *p < 0.05, **p < 0.01, or ****p < 0.0001, and “ns” indicates non-significance compared with the control (blank). Data are representative of three independent experiments.