Table 2 Primers used in this study
Primer | Sequence (restriction enzyme sites are underlined) | Restriction enzyme |
|---|---|---|
LI0666-F | ATTACAAGGATGACGATGACAATGGCGGAGGAGCGGCCGCGATGAAAATTCAATTTAAA | NotI |
LI0666-R | GCGTGAATGTAAGCGTGACATAACTAATTACATGACTCGAGTTAGTTATTCTTCCCTTT | XhoI |
LI0666Y149A-F | AACCCTTCATCAGAGCCTATTGCTGCAGAACTTGATTTTAC | Fusion PCR |
LI0666Y149A-R | TGCAGCAATAGGCTCTGATGAAGGGTT | |
LI0666Y186A-F | CCACATGTAACAGAACCCATTGCTGCTGAAATTAAAACAAC | |
LI0666Y186A-R | AGCAGCAATGGGTTCTGTTACATGTGG | |
pBAD24-YopE-F | CCGGAATTCATGAAAATATCATCATTT | EcoRI |
pBAD24-YopE-HA-R | CCCAAGCTTAAGCGTAATCTGGTACGTCGTATGGGTAGTCGACCATCAATGACAGTAA | HindIII SalI |
pBAD24-RplJ-F | GTTTTTTTGGGCTAGCAGGAGGAATTCATGGCACTAAATCTTCAA | EcoRI |
pBAD24-RplJ-HA-R | ATCTGGTACGTCGTATGGGTAGTCGACAGCAGCTTCTTTCTGATC | SalI |
pBAD24-LI0666-F | GTTTTTTTGGGCTAGCAGGAGGAATTCATGAAAATTCAATTTAAA | EcoRI |
pBAD24-LI0666-HA-R | ATCTGGTACGTCGTATGGGTAGTCGACGTTATTCTTCCCTTTATT | SalI |
pBAD24-LI0666N40-R | GCTAGATCCTGACACAGAGCTCTCTTTTTTAGTAGG | |
EGFP-F | ccgGAATTCTGGCAGGTGCTGGTGCTGGTGCTGGAGCAATCCTGGTGAGCAAGGGCGAG | EcoRI |
EGFP-R | ccgGACTCGAGCTTACTTGTACAGCTCGTC | XhoI |
LI0666∆N39-EGFP-F | CGCAGGATCCAGGGAGTAGAAGAAAATGGT | BamHI |
LI0666∆C64-EGFP-R | AGCACCTGCCAGAATTCCTTCTGGAATAGGAGGTAACG | EcoRI |
LI0666-EGFP-F | AAGGTACCTAGGATCCAGATGAAAATTCAATTTAAA | BamHI |
LI0666-EGFP-R | AGCACCTGCCAGAATTCCGTTATTCTTCCCTTTATT | EcoRI |
pEGFP-C1-LI0666-F | ATGGATGAGCTGTACAAGTCCGGACTCAGATCTCGAGCTATGAAAATTCAATTTAAA | XhoI |
pEGFP-C1-LI0666-R | ATGGCTGATTATGATCAGTTATCTAGATCCGGTGGATCCTTAGTTATTCTTCCCTTT | BamHI |
pEGFP-C1-LI0666∆C64-R | CGCGGATCCTTATTCTGGAATAGGAGGTAACG | BamHI |