Figure 4

PPV infection induces Raptor phosphorylation by activating AMPK phosphorylation. A, B PTCs were infected with PPV or mock for 12 h. Raptor and p-Raptor were evaluated via Western blotting (A), and the ratio of p-Raptor/Raptor in cells was calculated and analysed (B). C–E PTCs were pre-treated with Compound C (10 μM) and then infected with PPV (MOI = 1) for 12 h. The levels of the cellular proteins indicated were evaluated by Western blotting (C), and the ratios of p-AMPK to AMPK and p-Raptor to Raptor as reported in D, E were calculated and analysed. The results are presented as the mean ± SD of three experiments, * p < 0.05; ** p < 0.01.