Table 2 Primer sequences used for real-time PCR
Genes and GenBank accession numbers | Primer sequence (5′–3′) | Fragment size (base pairs) |
|---|---|---|
Nrf2 (MN416129.1) | AGCAGTGAATAGCAACACCAGTCC CGAGGCTGCTGTCTGTATCTGAAG | 125 |
HO-1 (X56201.1) | GATGCGTTCTGGCGGTGCTC GCTGTCGGTGCTGTTGCTCTG | 147 |
GCLC (XM_419910.5) | GGAGAGGCGGTGTAAGAGAAGAGG GACTGTGGGAGCAGCAGCAATG | 137 |
GCLM (NM_001007953.1) | GCTGCTAACTCACAATGACC TGCATGATATAGCCTTTGGAC | 127 |
GPx1 (NM_001277853.2) | GACCAACCCGCAGTACATCA GAGGTGCGGGCTTTCCTTTA | 112 |
NQO1 (NM_001277621.1) | AAGGGCTGGGAAGTCACCAT CGTAGACAAAGCACTCGGGG | 107 |
ChTLR15 (FJ915250.1) | GGCTGTGGTATGTGAGAATG ATCGTGCTCGCTGTATGA | 155 |
MyD88 (NM_001030962.4) | GCCTCGGCCTTTACCTCAAC CCGGATCTCCAGGTAGTCGT | 92 |
NF-κB (M86930.1) | TCTGAACAGCAAGTCATCCATAACG AAGGAAGTGAGGTTGAGGAGTCG | 127 |
NLRP3 (KF318520.1) | GGTTTACCAGGGGAAATGAGG TTGTGCTTCCAGATGCCGT | 114 |
IL-10 (AJ621254.1) | CAGCACCAGTCATCAGCAGCAGAGC GCAGGTGAAGAAGCGGTGACAG | 94 |
IL-18 (GU119895.1) | ATCGCAGTGTGTGCAGTACG ACGAACCACAAGCAACTGGC | 141 |
IL-1β (HQ739080.1) | CAGTGTGTGCAGTACGGCTT ACGCTGAATGCAACAGGCAT | 268 |
GAPDH (JQ280469.1) | GACGTGCAGCAGGAACACTA ATGGCCACCACTTGGACTTT | 129 |