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Figure 6 | Veterinary Research

Figure 6

From: A multifunctional enolase mediates cytoadhesion and interaction with host plasminogen and fibronectin in Mycoplasma hyorhinis

Figure 6

Interaction of rEno and its mutants with fibronectin. A Far-Western blotting analysis. WT rEno, single mutants K451L and K452L, and double mutant K451L-K452L were transferred to a PVDF membrane and incubated with fibronectin and anti-fibronectin antibody. BSA served as a negative control. Protein bands were visualized using ECL substrate. B SPR analysis. rEno and the three different mutants were injected over immobilized fibronectin. Sensorgrams depict the binding of immobilized fibronectin to rEno and the three different mutants. The arrow indicates the end of the injection period, at which point dissociation of rEno and the three different mutants from fibronectin can be observed. Different proteins are represented by different colored lines. RU, resonance units.

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Veterinary Research

ISSN: 1297-9716