Figure 1

Generation of bovine 3D-derived 2D enteroids. Representative brightfield images of (A) crypts directly after isolation from distal ileum of a 6-month-old calf, B Matrigel-embedded crypts form villus-crypts structures by day 7 of culture. Data is representative of six independent experiments using six individual calves. Enteroids were passaged three times and on day 5 of culture 3D enteroids were C mechanically disrupted by vigorous pipetting and D enzymatically dissociated into single cells using TrypLE Express. E Representative DIC image of the morphology of 2D enteroid cultured for 3 days at 2.5 × 10⁴ cells/well on 2% Matrigel coated 96-well plate in complete IntestiCult mouse media supplemented with 20% FBS. F Representative brightfield image of 2D enteroids at day 10 of culture. Data is representative of three independent experiments using three individual calves.