Table 1 Histopathological and Immunohistochemical scores and distribution in naturally infected guinea fowls, chickens and ducks infected with H5 HPAIVs
Histopathology (lesion frequency and average score*) | Immunohistochemistry** (average intensity and distribution) | ||||||||
|---|---|---|---|---|---|---|---|---|---|
Species Dead Euthanized | Guinea fowl n = 9 – | Chicken n = 9 n = 7 | Duck n = 8 n = 2 | Guinea fowl | Chicken | Duck | |||
Lung | 100% (1.7) – | 89% (1.3) 100% (1.3) | 25% (0.4) 100% (1.0) | +++ | Air capillaries, endothelial cells, leukocytes | ++ | Air capillaries, endothelial cells | + | Leukocytes, epithelial bronchial cells |
Trachea | 67% (1.2) – | 56% (0.7) 86% (1.0) | 57% (0.7) 0% (0) | +++ | Endothelial cells, epithelial cells | ++ | Endothelial cells | + | Epithelium and necrotic debris |
Spleen | 100% (2.4) – | 100% (1.8) 100% (1.7) | 100% (1.0) 100% (1.0) | +++ | endothelial cells, leukocytes | ++ | endothelial cells, leukocytes | + | Lleukocytes |
Liver | 22% (0.4) – | 22% (0.4) 0% (0) | 0% (0) 0% (0) | ++ | Cholangiocytes, endothelial cells | NE | − | – | |
Heart | 50% (0.5) – | 0% (0) 33% (0.3) | 38% (0.4) 0% (0) | + | Endothelial cells | + | Endothelial cells, mesothelial cells | + | Leukocytes |
Kidney | 11% (0.1) – | 0% (0) 0% (0) | 0% (0) 0% (0) | ++ | Endothelial cells | NE | − | – | |
Brain | 0% (0) – | 67% (1.3) 57% (1.1) | 14% (0.1) 0% (0) | ++ | Endothelial cells | ++ | Neurons, glial cells, necrotic debris | – | – |
Pancreas | 0% (0) – | 0% (0) 0% (0) | 0% (0) 0% (0) | + | endothelial cells | ++ | Endothelial cells | − | – |
Proventriculus | 0% (0) – | 56% (0.7) 60% (1.0) | 0% (0) 0% (0) | + | endothelial cells | ++ | Endothelial cells | NA | |
Intestine | 67% (1.0) – | 67% (1.0) 71% (1.0) | 0% (0) 50% (0.5) | ++ | Endothelial cells, leukocytes, epithelium | ++ | Endothelial cells, leukocytes, (epithelium) | + | Epithelium, leukocyte |