Figure 4
Histopathological findings and viral antigen detection in H5 HPAIV naturally infected guinea fowls. A Lung, mixed leukocytic interstitial pneumonia. Widespread NP-positive cells expanding air capillaries. B Trachea, epithelial degeneration and congestion. Widespread NP-positive cells in endothelial and epithelial cells. C Spleen, necrotizing splenitis with thrombosis. Widespread NP-positive endothelial cells and leukocytes. D, Cecum, disseminated single cells necrosis/apoptosis within lamina propria and epithelium. Frequent NP-positive cells within epithelium. E, Brain, within normal limits. Frequent NP-positive endothelial cells. F Heart, mild congestion, mild heterophilic infiltration. Sparse NP-positive cells in endothelial cells. G Liver bile duct, cholangiocellular degeneration and necrosis with sloughing and luminal cellular debris. Frequent multifocal viral antigen staining in epithelial cells of bile ducts and within luminal necrotic debris. H Bronchus, lymphoplasmacytic infiltration of lamina propria. Abundant NP-positive endothelial, epithelials cells and leukocytes. Hematoxylin and eosin (HE). Anti-nucleoprotein immunohistochemistry (IHC).