Figure 6

The N-terminal domain of ASFV MGF360-11L exerted its inhibitory function. A HEK-293 T cells were co-transfected with IFN-β-Luc (100 ng), ISRE-Luc (100 ng), pRL-TK (10 ng), cGAS (100 ng), and STING (100 ng) plasmids, full-length MGF360-11L and its truncated plasmids (MGF360-11L-1, MGF360-11L-2) for 24 h, and cell lysates were used for dual-luciferase reporter assays. B, C HEK-293T cells were co-transfected with TBK1 (1 μg) or IRF7 (1 μg) plasmid, along with full-length MGF360-11L (1 μg) plasmid and its truncated plasmids (MGF360-11L-1, MGF360-11L-2) for 24 h. Cells were analyzed by Co-IP and Western blotting with the indicated antibodies. D cGAS (500 ng) and STING (500 ng) plasmids were co-transfected into HEK-293T cells, along with the MGF360-11L-2 (1 μg) plasmid. Twenty-four hours post-transfection, RT–PCR was performed to determine IFN-β, ISG15, and ISG56 mRNA levels. All experiments were independently repeated at least 3 times. The data are shown as the mean ± SD; n = 3. *p < 0.05, **p < 0.01, ***p < 0.001. IB, immunoblotting; HA, anti-HA-tagged monoclonal antibody; IP, immunoprecipitation; WCL, whole cell lysate. 11L: MGF360-11L; 11L-1: MGF360-11L-1 (1-180 aa); 11L-2: MGF360-11L-2 (167-353 aa).