Figure 1

ASFV MGF360-11L inhibited the activation of IFN-β and the ISRE promoter. HEK-293 T cells were co-transfected with IFN-β-Luc (100 ng), ISRE-Luc (100 ng), and pRL-TK (10 ng) plasmids and the IFN-I signaling molecule plasmids cGAS (100 ng, A), STING (100 ng, A), STING (200 ng, B), TBK1 (200 ng, C), IKKε (200 ng, D), IRF3-5D (200 ng, E), and IRF7 (200 ng, F), along with increasing doses of MGF360-11L (50, 100, 200 ng) plasmid or empty vector plasmid (pCMV-N-HA). 24 h post-transfection, cell lysates were used for dual-luciferase reporter assays. The expression of cGAS, STING, TBK1, IKKε, IRF, IRF7 and MGF360-11L was analyzed by Western blotting. All assays were independently repeated at least three times. The data are shown as the mean ± SD; n = 3. *p < 0.05, **p < 0.01, ***p < 0.001. Luc: luciferase.