Figure 8

Western blot analysis of the cross-reactivity of antisera elicited by rPhoE immunization with outer membrane proteins from other Enterobacteriaceae species. The outer membrane proteins extracted from E. coli, KP and P. aeruginosa were subjected to Western blot analysis using the anti-rPhoE mouse serum as the primary antibody, and an HRP-conjugated goat anti-mouse IgG as the secondary antibody. Positive bands with approximately 38 kDa correspond to the size of PhoE (lane 1: KP, lines 2 and 3: E. coli). No cross-reactivity was observed for P. aeruginosa.