Figure 6
PhoE inoculation induced protection from KP challenge and reduced the visceral bacterial load. A Summary of the experimental scheme of organ recovery and survival assay. B BALB/c mice (n = 10/group) were immunized with PhoE, IWB or mock-immunized with PBS and challenged with 5 × 106 CFU of CVCC4080 through intraperitoneal injection. The general condition of the animals was recorded every 12 h. Significance was calculated by comparing survival of the mice immunized with PhoE and mock-immunized with PBS using the Log-Rank Mantel Cox test. C, D The number of bacteria in visceral organs was determined 5 days after infection (n = 5/group) by spreading the organ homogenates on LB agar plates. **p < 0.01.