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Table 2 Primer sequences used in the expression of the His-MsrA fusion protein

From: Luteolin increases susceptibility to macrolides by inhibiting MsrA efflux pump in Trueperella pyogenes

Gene

Orientation

Sequence (5′-3′)

Restriction site

msrA

Forward

GGATCCATGGAACAATATACAATTAAATTTAACCAAATCA

BamH I

Reverse

AAGCTTTTAAGTTATATCATGAATAGATTGTCCTGTTAATTCC

Hind III

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Veterinary Research

ISSN: 1297-9716