Route of infectious bronchitis virus vaccination determines the type and magnitude of immune responses in table egg laying hens

Table 1 Details of primers used in the qRT-PCR analysis of gene signatures, interferons and cytokines

Gene group	Gene target	Primer sequences: forward (F) and reverse (R)	Reference
Reference gene	18S ribosomal RNA	(F) TGTGCCGCTAGAGGTGAAATT	[14]; [41]
Reference gene	18S ribosomal RNA	(R) TGGCAAATGCTTTCGCTTT	[14]; [41]
Cellular immune response	CD8-α	(F) TTG GAC GGG ACC TTA CAG AC	[14]
	CD8-α	(R) TGA AGG GAG CAA AGG AGA AA	[14]
	CD8-β	(F) CTGCATGGCTCCGACAATGG	[40]
	CD8-β	(R) ATCGACCACGTCAAGCTGGG	[40]
Mucosal immune response	ChIgA (chicken immunoglobulin A)	(F) TGCAGGGCAATGAGTTCGTCTGTA	[42]
	ChIgA (chicken immunoglobulin A)	(R) AGGAGGTCACTTTGGAGGTGAAT	[42]
	ChIgY (chicken immunoglobulin G)	(F) GACGAAGCTT TTCCTCTTCT	[43]
	ChIgY (chicken immunoglobulin G)	(R) CCCGATTGTA CCCTCTATCG	[43]
Viral recognition	TLR3 (toll-like receptor 3)	(F) GCAATTTCTCCTTCACCTTTTCA	[44]
	TLR3 (toll-like receptor 3)	(R) CCTTTATGTTTGCTATGTTGTTATTGCT	[44]
	MDA5 (melanoma differentiation-associated protein 5)	(F) AGGAGGACGACCACGATCTCT	[44]
	MDA5 (melanoma differentiation-associated protein 5)	(R) CCACCTGTCTGGTCTGCATGT	[44]
Interferon	IFN-β [interferon beta (type I)]	(F) TCCAACACCTCTTCAACATGCT	[44]
Interferon	IFN-β [interferon beta (type I)]	(R) TGGCGTGTGCGGTCAAT	[44]
Inflammation	IL-6 (interleukin 6)	(F) CACGATCCGGCAGATGGT	[44]
Inflammation	IL-6 (interleukin 6)	(R) TGGGCGGCCGAGTCT	[44]

ISSN: 1297-9716