Table 1 Strains and plasmids used in this study

Strains

 E. coli

  DH5α

Clone host strain, supE44 ∆lacU169(ϕ80 lacZ∆M15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1

Invitrogen

  BL21

Expression strain, F-ompT hsdS(rB- mB-) gal dcm (DE3)

Invitrogen

  WT

MPEC (DCM5), wild-type

Laboratory stock

  XW10

DCM5 lsrR-deletion mutant

This study

  WT/pSTV28

WT with the empty vector pSTV28, Cm^r

This study

  XW10/pSTV28

XW10 with the empty vector pSTV28, Cm^r

This study

  XW10/pClsrR

XW10 with the complement plasmid pClsrR, Cm^r

This study

  WT/pUC19

WT with the empty vector pUC19, Amp^r

This study

  WT/pUClsrR

WT with the overexpression plasmid

pUClsrR, Amp^r

This study

  WTΔlacZ

DCM5 lacZ-deletion mutant

This study

  XW10ΔlacZ

DCM5 lacZ and lsrR mutant strain

This study

  WTΔlacZ/pRCL-p_ahpCF

WTΔlacZ with plasmid pRCL-p_ahpCF, Cm^r

This study

  XW10ΔlacZ/pRCL-p_ahpCF

XW10ΔlacZ with plasmid pRCL-p_ahpCF, Cm^r

This study

  WTΔlacZ/pRCL-p_oxyR

WTΔlacZ with plasmid pRCL-p_oxyR, Cm^r

This study

  XW10ΔlacZ/pRCL-p_oxyR

XW10ΔlacZ with plasmid pRCL-p_oxyR, Cm^r

This study

  WTΔlacZ/pRCL-p_katG

WTΔlacZ with plasmid pRCL-p_katG, Cm^r

This study

  XW10ΔlacZ/pRCL-p_katG

XW10ΔlacZ with plasmid pRCL-p_katG, Cm^r

This study

  WTΔlacZ/pRCL-p_katE

WTΔlacZ with plasmid pRCL-p_katE, Cm^r

This study

  XW10ΔlacZ/pRCL-p_katE

XW10ΔlacZ with plasmid pRCL-p_katE, Cm^r

This study

  WTΔlacZ/pRCL-p_yciF

WTΔlacZ with plasmid pRCL-p_yciF, Cm^r

This study

  XW10ΔlacZ/pRCL-p_yciF

XW10ΔlacZ with plasmid pRCL-p_yciF, Cm^r

This study

  WTΔlacZ/pRCL-p_rpoS

WTΔlacZ with plasmid pRCL-p_rpoS, Cm^r

This study

  XW10ΔlacZ/pRCL-p_rpoS

XW10ΔlacZ with plasmid pRCL-p_rpoS, Cm^r

This study

  WTΔlacZ/pRCL-p_katGM6

WTΔlacZ with plasmid pRCL-p_katGM6, Cm^r

This study

  WTΔlacZ/pRCL-p_ahpCFM6

WTΔlacZ with plasmid pRCL-p_ahpCFM6, Cm^r

This study

 Plasmids

  pKD46

Expresses λ red recombinase Exo, Bet and Gam, temperature sensitive, Amp^r

[31]

  pKD3

cat gene, template plasmid, Amp^r Cm^r

[31]

  pCP20

FLP  +  λcI857  +  λpRRep(Ts), temperature sensitive, Amp^r Cm^r

[31]

  pSTV28

Low copy number cloning vector, Cm^r

Takara

  pUC19

Cloning vector, Amp^r

Takara

  pUClsrR

pUC19 with lsrR gene, Amp^r

This study

  pClsrR

pSTV28 with lsrR gene, Cmr

This study

  pRCL

Cm^r, promoterless lacZ

[46]

  pRCL-p_ahpCF

pRCL harboring ahpCF promoter

This study

  pRCL-p_oxyR

pRCL harboring oxyR promoter

This study

  pRCL-p_katG

pRCL harboring katG promoter

This study

  pRCL-p_katE

pRCL harboring katE promoter

This study

  pRCL-p_yciF

pRCL harboring yciF promoter

This study

  pRCL-p_rpoS

pRCL harboring rpoS promoter

This study

  pRCL-p_katGM6

Mutational pRCL-p_katG with 6-bp mutation (‘AACAAT’ to ‘GCGCGC’)

This study

  pRCL-p_ahpCFM6

Mutational pRCL-p_ahpCF with 6-bp mutation (‘AAAACT’ to ‘GCGCGC’)

This study

  pET28a (+)

Expression vector, Kan^r

Novagen

  pET-lsrR

pET28a (+) with lsrR gene, Kan^r

This study