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Table 3 Loop-mediated isothermal amplification (LAMP) absorbance time thresholds with different combinations of PM, MH, and HS gDNA

From: On-farm colorimetric detection of Pasteurella multocida, Mannheimia haemolytica, and Histophilus somni in crude bovine nasal samples

  1. Data illustrating the time (in minutes) obtained from Figure 4 and the samples in which the colorimetric absorbance threshold (430 nm/520 nm) of 3.0 was crossed for each combination of Pasteurella multocida (PM), Mannheimia haemolytica (MH), and Histophilus somni (HS) in the quantitative LAMP (qLAMP) assay shown in Additional file 12. Primer set kmt1 detects PM, rsmL detects MH and lolB detects HS. A value of 60 indicates that the colorimetric absorbance ratio did not reach the 3.0 threshold. The values highlighted in blue are less than or equal to the time threshold for the respective primer set and hence considered positive amplification.
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Veterinary Research

ISSN: 1297-9716