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Figure 3 | Veterinary Research

Figure 3

From: Genetic interference exerted by Salmonella-delivered CRISPR/Cas9 significantly reduces the pathological burden caused by Marek’s disease virus in chickens

Figure 3

In vitro plasmid delivery efficacy. A Caco2 cells were transfected with the CRISPR/Cas9 plasmid via either lipofection with variable plasmid concentrations (5, 10, or 15 μg/well) or ST-mediated bacterial transfection with variable MOIs (100 and 150) and observed using an IncuCyte live imaging system for six days. Images acquired on Day 4 are shown. The development of green fluorescent objects was observed and quantified. B The number of green objects per image per well and the confluence levels measured over six days are depicted. Two independent trials were performed, and representative images are shown. The arrow indicates the time of plasmid introduction or ST infection.

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Veterinary Research

ISSN: 1297-9716