Table 1 Primers used for amplification of NKIRAS2 and TNFAIP3 for psiCHECK™-2 recombinant vector construction.
From: MiR-125b regulates inflammation in bovine mammary epithelial cells by targeting the NKIRAS2 gene
Primer name | Primer sequence (5′-3′) | Size (bp) |
|---|---|---|
NKIRAS2-wt | F: 5′ TTCCGCTCGAGTGGGGTAGTTGTGGGTCATT 3′ R: 5′ AATACTAAGCGGCCGCCATTTCATTCCACCTCACCC 3′ | 791 |
NKIRAS2-mut | F: 5′ TGTAACTCTTCCCCTAGCTTCCTTTTCCTGCTTAG 3′ R: 5′ TGACATGCTCCCTAAGCAGGAAAAGGAAGC 3′ | – |
TNFAIP3-wt | F: 5′ CTTGTGCCCTCGAGAAACCCAGAGCCAGTCCC 3′ R: 5′ TTATCATTGCGGCCGCGCTCAGCCTCAGCATCAC 3′ | 270 |