Figure 5
From: MiR-125b regulates inflammation in bovine mammary epithelial cells by targeting the NKIRAS2 gene
Bovine miR-125b regulates the expression of NF-κB, TNF-α and IL-6 in LPS-induced MAC-T cells. The MAC-T cells were transfected with miR-125b mimic, inhibitor or scrambled control (NC, Cy3-labeled). The transfection efficiency of bovine miR-125b mimic or inhibitor in MAC-T cells as measured by fluorescence micrograph (A) and qPCR (B). At 48 h post-transfection, MAC-T cells were challenged with LPS for 3 h. Cells were then collected to measure mRNA expression and production of NKIRAS2 (C), NF-κB (D), IL-6 (E), and TNF-α (F). Expression levels of mRNA and protein were determined by qPCR and Western blot, respectively. *p < 0.05, **p < 0.01, ***p < 0.001.