Figure 3
From: MiR-125b regulates inflammation in bovine mammary epithelial cells by targeting the NKIRAS2 gene
The tumor necrosis factor alpha-induced protein 3 (TNFAIP3) gene is not directly targeted by bovine miR-125b. A The sequence alignments of miR-125b and its putative targets in the 3′ untranslated region (UTR) of TNFAIP3, which have been identified as targets of miR-125b in human (has-miR-125b) [8, 12]. B Human embryonic kidney (HEK) 293 T cells were transiently co-transfected with CHECK2-3′ UTR dual-luciferase reporter recombinant vector, and bovine miR-125b mimic or NC_mimic. The data in legend are the relative luciferase activity (Renilla / Firefly), in which, renilla luciferase activity was normalized to the activity of firefly luciferase in the same recombination vector. Data are Mean ± SD. ns: adjusted p value > 0.05, as determined by one-way ANOVA multiple comparisons. NC_mimic = negative control, compared with miR-125b mimics; CHECK2-3′UTR = TNFAIP3-3UTR-wt and psiCHECK™-2 dual-luciferase reporter recombinant vector.