Skip to main content
Figure 6 | Veterinary Research

Figure 6

From: Generation of equine enteroids and enteroid-derived 2D monolayers that are responsive to microbial mimics

Figure 6

Effects of TLR agonist on cytokine gene expression in equine enteroid-derived 2D monolayer cultures. The relative gene expression of TNF-α (A), IL-8 (B), TGF-β (C), IL-33 (D) and IFN-β (E) in monolayer cultures exposed to Pam3CSK4, lipofected Poly I:C, LPS or FliC for 20 h. The cytokine gene expression was normalised to the geometric mean for the reference genes (HPRT, SDHA and GAPDH) and calibrated to that in the medium control. The results are given as mean values ± SD for cultures established from two of the experimental horses (n = 2). FC > 2 shown by dashed line indicates gene up-regulation. nd = not detected.

Back to article page

Veterinary Research

ISSN: 1297-9716