Table 4 Differentially expressed genes in testicle tissues of experimentally and naturally infected males
Analyzed tissue | Gene regulation | Intravenously inoculated group | Subcutaneously inoculated group | Intradermally inoculated group |
|---|---|---|---|---|
Experimentally infected calves | ||||
Scrotal skin | Upregulation | CCL24 | No significant changes | No significant changes |
Downregulation | IL10; TLR2 | IL6; IL8; IL10; SERP; SELE; PLAT; ICAM1; ADAMTS1; IL4; IL17; TGF; TNF | IL6; IL8; IL10; IL1a; TLR2; SELE; PLAT; ICAM1; VCAM1; CCL2; CXCL2; TIMP1; ADAMTS1; IL17; TGF; TNF | |
Pampiniform plexus | Upregulation | CCL24 | No significant changes | CCL24 |
Downregulation | IL6; ADAMTS1 | IL6; IL8; IL10; IL1a; SERP; PLAT; CCL2; ADAMTS1; TIMP1; IL17 | IL6; IL8; IL10; IL1a; TLR2; SERP; SELE; PLAT; ICAM1; VCAM1; CCL2; TIMP1; ADAMTS1; IL17; TGF; TNF | |
Testicular parenchyma | Upregulation | No significant changes | CXCL2 | CCL24 |
Downregulation | IL6; IL8; IL10; SERP; CCL2; ADAMTS1; TIMP1 | IL6; IL8; SERP; PLAT; CCL2; ADAMTS1; IL17; TGF | IL6; IL8; IL10; SELE; PLAT; VCAM1; TIMP1; IL17; TLR2; TNF; TGF | |
Analyzed tissue | Gene regulation | Acutely infected bulls without tissue cysts | Acutely infected bulls with tissue cysts | Chronically infected bulls |
|---|---|---|---|---|
Naturally infected animals | ||||
Scrotal skin | Upregulation | No significant changes | No significant changes | ICAM1 |
Downregulation | No significant changes | No significant changes | PLAT; IL8 | |
Pampiniform plexus | Upregulation | PLAT; IL1a | PLAT; TLR2 | ICAM1 |
Downregulation | No significant changes | No significant changes | PLAT; MMP13 | |
Testicular parenchyma | Upregulation | PLAT; IL1a; MMP13; TIMP1; IL6 | IL1a; TIMP1 | ICAM1 |
Downregulation | IL8; IL4; CCL24 | MMP13; IL8; CCL24 | PLAT; IL8; MMP13; IL1a; IL6; IL4; TGFB1; CCL24 | |