Figure 5

Circulating Mycoplasma hyopneumoniae-specific cytokine producing T-cell subsets and IFN-γ production in blood of neonatal piglets. A–C Circulating Mycoplasma hyopneumoniae-specific cytokine producing T-cell subsets in neonatal piglets; D IFN-γ production by CD3⁻ cells; E IFN-γ production in different T-cell subsets. PBMCs were stimulated with M. hyopneumoniae J strain bacterin and T-cell phenotype and cytokine production were assessed by flow cytometry. Farm A: n = 23 piglets, endemically infected with M. hyopneumoniae; Farm B: n = 24 piglets, free of M. hyopneumoniae. On farm A, sows were vaccinated against M. hyopneumoniae at 6 and 3 weeks before farrowing, while sows on farm B were not vaccinated. *, P < 0.05 between farm A and B.