Figure 1

Specificity of mAbs by confocal laser scanning microscopy and flow cytometry (FCM). A 3D4/21 cells were infected with G. parasuis-5, six mAbs were added to the cell wells as primary antibodies and incubated at 37 °C for 1 h, and goat anti-mouse IgM/FITC antibody was used as the secondary antibody. After washing with PBS 3 times, the coverslips were placed upside down on clean glass slides dripped with 10 μL of 50% glycerol and mounted and observed under a laser confocal microscope. Fluorescence was observed in the cell wells with G. parasuis-5 and mAbs 1A12, 3E3, 4C6, 2D1, 3E6, and 4B2, and there was no fluorescence in the cell wells with G. parasuis-5 and the negative control. Scale bar = 5 μm. B G. parasuis-5 cells were labeled with a CFDA-SE cell proliferation and tracer detection kit. FSC and SSC were used to set up the gate to delineate the area of G. parasuis-5 in the scatter diagram. Six mAbs were used as primary antibodies, and goat anti-mouse IgM/FITC was used as the secondary antibody. They were all incubated with G. parasuis-5 and then used for FCM analysis. Fluorescence was observed in G. parasuis-5 incubated with six mAbs, but there was no fluorescence in G. parasuis-5 incubated with the negative control.