Figure 5From: CRISPR/Cas9-based generation of a recombinant double-reporter pseudorabies virus and its characterization in vitro and in vivoThe replication and distribution of rPRV HN1201-EGFP-Luc in a mouse model. 6-week-old BALB/c mice were infected I.M. with 2% FBS DMEM or recombinant pseudorabies virus rPRV HN1201-EGFP-Luc (106.5, 105.5, 104.5, 103.5 or 102.5 TCID50 per mouse) in the left leg. Viral replication and distribution were quantified at the indicated times after infection by whole-animal bioluminescence imaging.Back to article page