Porcine enteroid monolayer development and interconvertibility with 3D culture. A Growth of jejunal crypts, plated on collagen-coated wells, using L-WRN-conditioned medium followed from days 1 to 7. B Comparison of 2D-monolayers from duodenum, jejunum and ileum at day 3 after passaging. C Interchange between 2 and 3D cultures and back of jejunal enteroids representative for 3 piglets. D 3D and (E) 2D-jejunal enteroid cultures were stained with anti-SOX9 or isotype control antibodies at days 7 and 3 respectively after passage. Images are representative for 2 piglets. Scale bar equals 100 µm.