Table 1 Primer sequences used in this study
Primer name | Primer sequence (5′–3′) | Usage |
|---|---|---|
TLR19-F | TGCATCGATGCTGAGTCGCTG | tlr19 cloning |
TLR19-R | GGCAGAGCTTCTCCATTGTGGCCA | tlr19 cloning |
TLR19-5′outer | AGCGAGCAAAGCGATAACCAGCGG | 5′RACE |
TLR19-5′inner | GCGATAACCAGCGGCTGCCAACAG | 5′RACE |
TLR19-3′outer | GGAACAAGGGAACCCGAGACTGA | 3′RACE |
TLR19-3′inner | GAGAGTATCCACAGCAGTCAGTGC | 3′RACE |
TLR19-NI-F | ACATCCTGCAGGAATGGGTGTGCATGACTCC | Plasmid construction |
TLR19-SI-R | CTAGCTAGCTCAAGAAGCTTCCGCGTC | Plasmid construction |
TLR19-EI-F | CGGAATTCATGGGTGTGCATGACTCC | Plasmid construction |
TLR19-SI-R | GGATTCCCTCAAGAAGCTTCCGCGTC | Plasmid construction |
rtTLR19-F | GCCGCTGGTTATCGCTTTGCT | Real-time PCR |
rtTLR19-R | ATCCTCCTGTGCCACTGCCTAC | Real-time PCR |
rtS11-F | CCGTGGGTGACATCGTTACA | Real-time PCR |
rtS11-R | TCAGGACATTGAACCTCACTGTCT | Real-time PCR |
Rab5-F | CCCAAGCTTATGGCAGGAAGAGGCGGA | Plasmid construction |
Rab5-R | CGGGGTACCGTGTTGCTACAGCGGGACC | Plasmid construction |
Rab7-F | CCCAAGCTTATGACATCAAGGAAGAAAGTTC | Plasmid construction |
Rab7-R | CGGGGTACCGTGCAGCTACAAGTCTCTGC | Plasmid construction |
TRIF-F | CCCAAGCTTCGCCACCATGGCAGATGGTGGAGTAGAG | Plasmid construction |
TRIF-R | CGCGGATCCCGAGAATCAAACCCATTGGGCGAG | Plasmid construction |
MyD88-F | CCCAAGCTTCGCCACCATGGCATCAAAATCAAGTATAGAC | Plasmid construction |
MyD88-R | CGCGGATCCCGTTGAAAAGATCGGGGCAGTGC | Plasmid construction |
TIRAP-F | CCCAAGCTTCGCCACCATGGAGGAAGACGCGTCAG | Plasmid construction |
TIRAP-R | CGCGGATCCCGGCCGTCAGATTGAGATGCAC | Plasmid construction |
EPC-ifn-1-F | ATGAAAACTCAAATGTGGACGTA | Real-time PCR |
EPC-ifn-1-R | GATAGTTTCCACCCATTTCCTTAA | Real-time PCR |
EPC-Viperin-F | AGCGAGGCTTACGACTTCTG | Real-time PCR |
EPC-Viperin-R | GCACCAACTCTCCCAGAAAA | Real-time PCR |
EPC-β-actin-F | GCCGTGACCTGACTGACTACCT | Real-time PCR |
EPC-β-actin-R | GCCACATAGCAGAGCTTCTCCTTG | Real-time PCR |