Effect of EP on tight junction proteins in the small intestine. A Western blot analysis of proteins from jejunal and ileal tissues probed with the anti-claudin-1, anti-occludin and anti-ZO-1 antibody. B–D. Results are presented as the ratio of target protein band intensity to the intensity of the β-actin band. Data are presented as the mean ± SEM. *P < 0.05; **P < 0.01.