Figure 1

Cumulative mortality and morbidity parameters in the evaluation of virulence degree in Toxoplasma gondii isolates. A Kaplan–Meier survival curves of Toxoplasma gondii isolates from sheep. ToxoDB# genotype of each isolate is indicated. B Clinical sign scoring used in the evaluation of morbidity. C Summary of the cumulative mortality and morbidity parameters in the evaluation of virulence degree. The gradation of clinical signs observed in mice inoculated with each isolate is shown. Coloured boxes represent the most severe clinical sign observed in each individual mouse challenged with each dose according to the clinical sign scoring included below. + : mice with a Toxoplasma-infection confirmed by IFAT or tissue imprints of brain or lung. − : mice without a confirmed Toxoplasma-infection. nd: not done. Cumulative mortality and morbidity rates are indicated. The dosages 10⁵, 10⁴,10³, 10², and 10¹ are included for cumulative mortality rate calculation, whereas all dosages were considered for cumulative morbidity rate calculation.