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Figure 2 | Veterinary Research

Figure 2

From: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) moonlights as an adhesin in Mycoplasma hyorhinis adhesion to epithelial cells as well as a plasminogen receptor mediating extracellular matrix degradation

Figure 2

Surface localization of GAPDH in M. hyorhinis. A Detection of GAPDH by flow cytometry. M. hyorhinis were incubated with anti-rGAPDH serum or negative serum respectively. M. hyorhinis treated with PBS was used as the blank control. The level of mean fluorescence intensity (MFI) of M. hyorhinis incubated with anti-rGAPDH serum is expressed as the percentage of the bacteria incubated with negative serum after deduction of the background (**P < 0.01). Data are expressed as mean ± SD of at least three experiments with samples in triplicate. B Detection of surface GAPDH by colony blot analysis. Reaction of M. hyorhinis colonies transferred to PVDF membrane was probed with anti-rGAPDH serum or negative serum.

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Veterinary Research

ISSN: 1297-9716