Figure 5From: Screening of potential vaccine candidates against pathogenic Brucella spp. using compositive reverse vaccinologyProtein structure and expression of Brucella antigens Omp19, VirB8, and HlyD. A–C The protein structure of the antigens Omp19, VirB8, and HlyD was generated with Phyre2 tools using a homology modelling approach. D–F SDS-PAGE and Western blot analysis of purified mature proteins. Omp19, VirB8, and HlyD were cloned as C-terminal His-tagged fusion proteins and then expressed and purified.Back to article page