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Figure 5 | Veterinary Research

Figure 5

From: Screening of potential vaccine candidates against pathogenic Brucella spp. using compositive reverse vaccinology

Figure 5

Protein structure and expression of Brucella antigens Omp19, VirB8, and HlyD. AC The protein structure of the antigens Omp19, VirB8, and HlyD was generated with Phyre2 tools using a homology modelling approach. D–F SDS-PAGE and Western blot analysis of purified mature proteins. Omp19, VirB8, and HlyD were cloned as C-terminal His-tagged fusion proteins and then expressed and purified.

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