Figure 3
Analysis of the role of GSDMD in producing cytokines/chemokines after S. aureus infection. WT and GSDMD−/− mice were infected s.c. with 1 × 107 CFU S. aureus, and abscess tissue was excised on day 2 post-infection. The homogenate supernatants of skins were detected for concentrations of the indicated cytokines and chemokines by ELISAs. A IL-1β, B IL-6, C TNF-α, D Ccl5, E Cxcl1. WT and GSDMD−/− BMDMs were treated with S. aureus for 24 h (MOI 1: 50). F–J Culture supernatants from BMDMs were analysed for IL-1β, IL-6, TNF-α, Ccl5, and Cxcl1 by ELISAs. A–E Data are shown as the mean ± SEM. n = 8 per group. Data were pooled from 2 independent experiments. F-J Data are shown as the mean ± SEM (three independent experiments) of each group (n = 3). Student’s t-test was performed. Statistical significance is indicated by *p < 0.05, **p < 0.01, and ***p < 0.001.