Figure 1

Effect of sodium selenite on SHK-1 cell viability and P. salmonis growth. A SHK-1 viability at different concentrations of sodium selenite supplemented in L-15 medium. B Bacterial growth of P. salmonis at different concentrations of sodium selenite supplemented in SRS broth. For A and B, experiments were performed for 12 days post-treatment (dpt). Each circle and square represent the mean ± SD of ten observations measured in at least two independent experiments. Bidirectional ANOVA and subsequent Bonferroni comparison test were performed with respect to the control (0 µM Na₂SeO₃). Colored asterisks indicate significant differences (p-value < 0.05) for de different condition. C Relative abundance (arbitrary units) and activity of glutathione peroxidase 1 (Gpx1). The glutathione peroxidase 1 experiment was carried out 24 h after treatment. The bars represent the means ± SD of the relative abundance measured in at least three independent experiments by qPCR, while the dispersion points show the glutathione peroxidase 1 activity as a result of 32 measures in at least two independent experiments. A Student t-test was performed for unpaired data between groups. Letters (a, A, b and B) indicate significant differences (p-value < 0.05).