Evaluation using latent class models of the diagnostic performances of three ELISA tests commercialized for the serological diagnosis of Coxiella burnetii infection in domestic ruminants

Table 2 Characteristics of the three ELISA tests used in the study

Name used in the current study	Test 1	Test 2	Test 3
Commercial name	IDEXX Q fever Ab test	LSIVetTM Ruminant Q fever Serum^a	ID.Vet ID Screen® Q fever indirect multi-species
Manufacturer	IDEXX	LSI Life Technologies^a	IDvet^b
Kit batches used in the current study	D401, E121	ELISACOXLS 020, ELISACOXLS 021, ELISACOXLS 023, ELISACOXLS 024	565 747
Strain used for antigen production	Isolated from Dermacentor andersoni ticks (Nine Mile reference strain)	Isolated from an ewe	Isolated from a cow
Conjugate	Secondary antibodies biding to ruminant IgG	Protein G (biding to IgG of diverse mammalian species)	Protein G (biding to IgG of diverse mammalian species)
Interpretation rules according to the manufacturer (ODR: optical density ratio)	ODR < 30% Negative 30% < ODR < 40% Doubtful 40% < ODR Positive	ODR < 40% Negative 40% < ODR < 100% Positive + 100% < ODR < 200% Positive + + 200% < ODR Positive + + +	ODR < 40% Negative 40% < ODR < 50% Doubtful 50% < ODR < 80% Positive 80% < ODR Strong positive

^aTest 2 is currently commercialized by Themofisher Scientific under the commercial name PrioCHECK™ Ruminant Q Fever Ab Plate Kit. ^bIDVet is now named Innovative Diagnostics.

ISSN: 1297-9716