Detection of apoptotic cells in neutrophils stimulated with M. bovis. Bovine neutrophils were evaluated at 1, 3, and 6 h after stimulation with M. bovis in five cows. The ratio of positive or negative neutrophils in Annexin V and/or 7-AAD is shown. Annexin V-positive/negative cells were defined as apoptotic/nonapoptotic cells, and 7-AAD–positive/negative cells were defined as dead/live cells. Data were expressed as means ± SE in five cows; significant difference at *P < 0.05 compared to unstimulated controls.