Figure 2
The growth curves of R. anatipestifer CH-1 and its fur mutant. A R. anatipestifer CH-1pLMF03, R. anatipestifer CH-1ΔfurpLMF03 and R. anatipestifer CH-1ΔfurpLMF03::fur cells were cultured overnight and then inoculated into 20 mL of GCB liquid medium at an OD600 of 0.05, the growth rates were determined at 37 °C with shaking cultivation (180 rpm) by measuring the OD600 every 2 h for 12 h. R. anatipestifer CH-1 (B) or R. anatipestifer CH-1Δfur (C) was precultured in iron-limited medium overnight and then inoculated into 20 mL of GCB or GCB supplemented with 50 μM EDDHA, 100 μM EDDHA and 200 μM EDDHA at an OD600 of 0.05, and the growth rates were monitored as above. The error bars represent the standard deviations of three independent experiments and three replicate samples for each experiment. Statistical significance was determined using two-way ANOVA (****P < 0.0001, ** P < 0.01).