Skip to main content

Table 1 Comparison between 2 and 3D cultures for generating liver in vitro liver models

From: Liver organoids in domestic animals: an expected promise for metabolic studies

  2D culture 3D culture
Morphology Monolayer Organized aggregates with multiple layers
Amplification Easy and convenient More complex
Stable long-term cultures
Differentiation Directed but limited on flat and surface
Constrained morphogenesis
Self-organization
Feasibility of mixed 3D structures of hepatoblast, mesenchymal and vascular cells
Genes and Proteins Hepatoblast and hepatocyte with embryonic phenotypes More mature hepatocytes—adult-like phenotypes
Advantages Fast and rapid establishment
Relatively inexpensive,
Well adapted to high throughput capacity
Partially mimicking the in vivo microenvironment
Reproducing the apical-basal polarity in multicellular structures
Control of factor gradient by microfluidic approaches
Disadvantages Flat surface
Automatic apical-basal polarity not mimicking the tissue structure
Costly and more laborious
High throughput capacity to be optimized
  1. Such analysis can be extrapolated for other organoid models as well.