Figure 7

Regulation of sopE by RyhB paralogs as determined by interaction site prediction and fluorescence assay. A Interaction site prediction between RyhB-1/RyhB-2 and sopE. 5′ UTR of sopE (nt 73–86) is predicted to form incomplete base pairing with RyhB-1 (nt 37–50) or RyhB-2 (nt 39–52). B prediction of sopE mRNA secondary structure by RNAstructure module of CLC Main Workbench(5.5) software. A hairpin loop is formed in 5′ UTR of sopE (nt 73–86) (marked in blue). C Single colonies on LB plates imaged using the natural light mode and fluorescence mode by an inverted fluorescence microscope. D Bacterial fluorescence values in LB liquid medium. E. GFP protein expression in E. coli TOP10 containing various fusion plasmids as determined by Western blotting. 1. no sRNA::5′ UTR sopE-gfp; 2. ryhB-1::5′ UTR sopE-gfp; 3. ryhB-2::5′ UTR sopE-gfp. GAPDH was used as a loading control.