Table 1 Bacterial strains and plasmids used in this study
Strains or plasmids | Description | Source |
|---|---|---|
Plasmids | ||
 pMC-Express | A broad host-range shuttle vector derived from pMIDG100, sodC promoter, Cmr | [27] |
 pRE112 | sacB mobRP4 R6K ori Cmr | [26] |
 pCZ51 | pRE112-ΔpcgD | This work |
 pCZ53 | pRE112-ΔhptE | This work |
 pCZ54 | Insertion of complete pcgD into pMC-Express | This work |
 pCZ56 | Insertion of complete hptE into pMC-Express | This work |
Strains | ||
 SM10 λ pir | E. coli thi thr-1 leu6 proA2 his-4 arg E2 lacY1 galK2, ara14xyl5 supE44, λ pir | [43] |
 ATCC25922 | E. coli, quality control strain for antibiotic sensitivity test | ATCC |
 PM0818 | P. multocida 0818, Wild-type and virulent, LPS genotype L1 | [10] |
 PMZ1 | PM0818 ΔpcgD::kanR | This work |
 PMZ3 | PM0818 ΔhptE::kanR | This work |