Table 2 PCR primers for constructing donor plasmid and detecting the recombinant virus
PCR products | Sequences of primers (5′–3′) |
|---|---|
HAL | F: GGTGACCTACTGACCCTCAACACC |
R: CAGCTCCTCGCCCTTGCTCACCATTGTTCCCGTTGGGGGA | |
EGFP | F: TCCCCCAACGGGAACAATGGTGAGCAAGGGCGAGGAGCTG |
R: CTTCTTTTAGGGGCCCGGAGCTTGTACAGCTCGTCCATG | |
fiber-2 + HAR | F: CATGGACGAGCTGTACAAGCTCCGGGCCCCTAAAAGAAG |
R: CTACTTTACCTGCATTTCGTCAG | |
Partial fiber-2 | F: CTCCAACTGGTTTGACCAGAACG |
R: GTCAAGCTGGGATGCTCTCACCATGC |