Figure 3

Phylogenetic analysis of PoCD9.1 and PoCD9.3. The phylogenetic tree was constructed with MEGA 6.0 software using the neighbour-joining method. For confidence values for the phylogenetic analysis, 1000 bootstrap trials were performed. The black triangle indicates the CD9 protein of Paralichthys olivaceus. The numbers at the forks indicate the bootstrap value. Species and their protein accession numbers are as follows: Seriola dumerili (XP_022610189.1), Lates calcarifer (XP_018519439.1), Sander lucioperca (XP_031149225.1), P. olivaceus CD9.1 (019,934,457.1), Salmo salar (XP_014003925.1), Salvelinus alpinus (XP_023827461.1), Danio rerio (NP_997784.1), Cyprinus carpio (KTF92709.1), P. olivaceus CD9.3 (XP_019964847.1), Nanorana parkeri (XP_018418195.1), Xenopus laevis (NP_001085461.1), Meleagris gallopavo (XP_031411372.1), Gallus gallus (NP_990093.1), Coturnix japonica (XP_015726988.1), Homo sapiens (NP_001760.1), Mustela putorius furo (XP_004766790.1), Microcebus murinus (XP_012616069.1), Mus musculus (NP_031683.1), Crassostrea gigas (XP_011430305.1), Mizuhopecten yessoensis (XP_021353101.1), Acanthaster planci (XP_022082820.1), Strongylocentrotus purpuratus (XP_030845089.1), Megachile rotundata (XP_003699639.1), and Linepithema humile (XP_012235518.1).