Skip to main content
Figure 2 | Veterinary Research

Figure 2

From: UL28 and UL33 homologs of Marek’s disease virus terminase complex involved in the regulation of cleavage and packaging of viral DNA are indispensable for replication in cultured cells

Figure 2

Construction and Identification of the UL28 and UL33 gene deletion and revertant constructs. A Schematic diagram represents a linear MDV genome consisting of a unique long (UL) region and a unique short (US) region. Both terminal repeats (TRL/TRS) and the internal repeat (IRL/IRS) regions locate on both sides of the UL and US region. Location of the UL28 and UL33 genes on MDV is also shown; B PCR analysis of the virus genome with UL28 and meq specific primers. The lanes 1, 2 and 3 indicate the PCR amplification of the UL28 gene using parental virus, MDV △UL28 or MDV △UL28-Re mutants as templates, respectively; The lanes 4, 5 and 6 indicate the PCR amplification of the Meq gene using parental virus, MDV △UL28 or MDV △UL28-Re mutants as templates, respectively; C PCR analysis of the virus genome with the UL33 and meq specific primers. The lanes 1, 2 and 3 indicate the PCR amplification of the UL28 gene using parental virus, MDV △UL33 or MDV △UL33-Re mutants as templates, respectively; the lanes 4, 5 and 6 indicate the PCR amplification of the Meq gene using parental virus, MDV △UL33 or MDV △UL33-Re mutants as templates, respectively; D RFLP analysis of the genomic DNAs of the parental virus, MDV △UL28 or MDV △UL28-Re. DNA was digested with EcoRV. lane 1 indicates the parental virus; lane 2 represents MDV △UL28; lane 3 indicates MDV △UL28-Re; E RFLP analysis of the genomic DNAs of the parental virus, MDV △UL33 or MDV △UL33-Re. DNA was digested with NdeI. lane 1 indicates the parental virus; lane 2 represents MDV △UL33; lane 3 indicates MDV △UL33-Re. The red arrow indicates the fragment size difference.

Back to article page

Veterinary Research

ISSN: 1297-9716