Table 1 Primer sequences for qRT-PCR
Gene | Primer Sequences (5′–3′) | Product length (bp) | Accession number |
|---|---|---|---|
IBV N | F: CAAGCTAGGTTTAAGCCAGGT | 218 | FJ904723.1 |
R: TCTGAAAACCGTAGCGGATAT | |||
TLR3 | F: ACCCGGATTGCAGTCTCAGTA | 95 | NM_001011691.3 |
R: CACTGTCCTTGCAGGCTGAG | |||
TLR7 | F: ACCGTCGCCTCAAGGAAGTCC | 145 | NM_001011688.2 |
R: ACGCAGTTGCACCTGAAGTCAATC | |||
MyD88 | F: AAGGTGTCGGAGGATGGTGGTC | 120 | NM_001030962.4 |
R: GGAATCAGCCGCTTGAGACGAG | |||
MDA5 | F: TCAGGAGGAGGACGACCACGAT | 168 | GU570144.1 |
R: TTCCCACGACTCTCAATAACAG | |||
IFN-α | F: GGACATGGCTCCCACACTAC | 75 | GU119896.1 |
R: TCCAGGATGGTGTCGTTGAAG | |||
IFN-β | F: GCCCACACACTCCAAAACACTG | 151 | GU119897.1 |
R: TTGATGCTGAGGTGAGCGTTG | |||
IFN-γ | F: CACTGACAAGTCAAAGCCGC | 87 | NM_205149.1 |
R: ACCTTCTTCACGCCATCAGG | |||
MHCI | F: GCCAACACGGACCAGCAGTAC | 81 | NM_001097530.1 |
R: GTCCAGGTTCTCGCGGTCAATC | |||
MHCII | F: GTTCTACCAGCGTTCGGAAGGC | 101 | DQ207939.1 |
R: TCTGAGCGGCGTCCAACTCC | |||
Fc receptor | F: TGTGAGGTGCGGACGGAGAG | 195 | AM412311.1 |
R: TCGGTGCCAGGAGAAGGAGATG | |||
MARCO | F: CACATAAGCGAGCCTCGAATCCAG | 81 | NM_204736.1 |
R: CAGCAGCAGCAGGTAGATGACAAG | |||
CD36 | F: ACCAGACCAGTAAGACCGTGAAGG | 154 | NM_001030731.1 |
R: ATGTCTAGGACTCCAGCCAGTGTG | |||
MIF | F: ATTGGCAAGATTGGAGGG | 127 | M95776.1 |
R: CGTTGGCAGCATTTATGTC | |||
CCL4 | F: GCAGTTGTTCTCGCTCTTC | 192 | NM_204720.1 |
R: GCGCTCCTTCTTTGTGAT | |||
K60 | F: GCTGCTGTCATGGCTCTT | 278 | Y14971.1 |
R: TTGGTGTCTGCCTTGTCC | |||
XCL1 | F: ATGAAACTCCACGCCACAGTT | 294 | NM_205046.1 |
R: TTATCTTCTTCTGGTAGTACG | |||
CXCL12 | F: TGTCGGAGGAGAAGCCTGTCAG | 158 | NM_204510.1 |
R: CACTTGCTTGCTGTTGCTCTTGAG | |||
IL-1β | F: TGGGCATCAAGGGCTACA | 244 | Y15006.1 |
R: TCGGGTTGGTTGGTGATG | |||
IL-6 | F: ATGGTGATAAATCCCGATGAAG | 153 | NM_204628.1 |
R: CCTCACGGTCTTCTCCATAAAC | |||
TNF-α | F: CAGATGGGAAGGGAATGAAC | 268 | NM_204267.1 |
R: AGAGCATCAACGCAAAAGGG | |||
NF-κB | F: TCAACGCAGGACCTAAAGACAT | 162 | M86930.1 |
R: GCAGATAGCCAAGTTCAGGATG | |||
iNOS | F: AGTGGTATGCTCTGCCTGCT | 171 | NM_204961.1 |
R: CCAGTCCCATTCTTCTTCC | |||
IL-10 | F: CAGCACCAGTCATCAGCAGAGC | 94 | NM_001004414.2 |
R: GCAGGTGAAGAAGCGGTGACAG | |||
PPAR-γ | F: GGGCGATCTTGACAGGAA | 175 | AB045597.1 |
R: GCCTCCACAGAGCGAAAC | |||
LC3II | F: GTACGAGAGCGAGAAGGACG | 83 | NM_001031461.1 |
R: AGACGGAAGATTGCACTCCG | |||
mTOR | F: CATGTCAGGCACTGTGTCTATTCTC | 77 | XM_417614.5 |
R: CTTTCGCCCTTGTTTCTTCACT | |||
Beclin-1 | F: TGGCTTTCTTGGACTGTGTG | 125 | NM_001006332.1 |
R: ACCACCACTGCCACCTGTAT | |||
Caspase-3 | F: CCACCGAGATACCGGACTGT | 176 | NM_204725.1 |
R: AACTGCTTCGCTTGCTGTGA | |||
Bax | F: ACTCTGCTGCTGCTCTCCTCTC | 174 | XM_025145468.1 |
R: ATCCACGCAGTGCCAGATGTAATC | |||
Bcl-2 | F: GAGTTCGGCGGCGTGATGTG | 92 | NM_205339.2 |
R: TTCAGGTACTCGGTCATCCAGGTG | |||
β-actin | F: ATTGCTGCGCTCGTTGTT | 173 | K02173.1 |
R: CTTTTGCTCTGGGCTTCA |