Figure 3

Macrophages activity assays. A CCK-8 assay. CCK-8 was used to detect the viability of HD11 cells and PBMCs-Mφ infected with IBV or treated with UV-IBV or PBS (Mock cells) from 12 to 48 hpi. B NO content assay. NO content of culture supernatants of IBV-infected, UV-IBV-treated or Mock cells were measured from 12 to 48 hpi. C, D Macrophage phagocytic function assay by flow cytometry. Phagocytic functions of chicken macrophages were detected at 24 hpi by phagocytosis of yellow-green fluorescent beads. Flow cytometry histogram shows fluorescent intensity of cells containing yellow-green fluorescent beads (green arrow) and cells not containing yellow-green fluorescent beads in three groups (C). The mean percentages of positive cells post-infection are shown in histograms (D). Data presented as means ± SD (n = 3). E Macrophage phagocytic function observation under fluorescent microscopy. Beads (green arrow) and nucleus (red) were observed under the inverted fluorescence microscope. Data presented as mean ± SD (n = 3). * means the significance of between IBV-infected or UV-IBV-treated cells with Mock cells. * means p < 0.05, ** means p < 0.01.