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Table 2 Oligonucleotide primers used for amplification of the second exon of the Ovar-DRB1 locus.

From: A novel technique for retrospective genetic analysis of the response to vaccination or infection using cell-free DNA from archived sheep serum and plasma

Primer name

Position relative to the first base of the second exon of the Ovar-DRB1 locus

Forward (F) or reverse (R)

Sequence

275

Intron 1 (−55 to −35)

F

ATTAGCCTCTCCCCAGGAGTC

329

Exon 2/intron 2 (263 to +15)

R

CACCCCCGCGCTCAC/CTCGCCGC

330

Intron 1 (−55 to −35)

F

ATTAGCCTCYCCCCAGGAGKC

455

Intron 1/exon 2 (−16 to +8)

F

TATCCCGTCTCTGCAG/CACATTTC

KBEH1

Intron 1/exon 2 (−7 to +14)

F

TCTGCAG/CACATTTCYTGGAG

  1. Intron 1 sequence is indicated as negative, intron 2 sequence is indicated as positive. Positions 1 to 270 indicate exon 2. The intron exon boundary is marked by /.