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Table 1 Bacterial strains and plasmids used in this study.

From: Identification of ireA, 0007, 0008, and 2235 as TonB-dependent receptors in the avian pathogenic Escherichia coli strain DE205B

Strain or plasmidCharacteristicsReferences
Strain
 DE205BO2:K1, NalR[21,22,23,24,25]
 DE205BΔireAireA Deletion mutant strain[21]
 DE205BΔireA/ireA*ireA Complemented strain[21]
 DE205BΔ00070007 Deletion mutant strainThis study
 DE205BΔ0007/0007*0007 Complemented strainThis study
 DE205BΔ00080008 Deletion mutant strainThis study
 DE205BΔ0008/0008*0008 Complemented strainThis study
 DE205BΔ22352235 Deletion mutant strainThis study
 DE205BΔ2235/2235*2235 Complemented strainThis study
 DE205BΔtonBtonB deletion mutant strainThis study
 DE205BΔtonB/tonB*tonB Complemented strainThis study
 DH5aCompetent invitrogen cellsVazyme
 BL21Competent invitrogen cellsVazyme
Plasmid
 pKD46Amp, expresses λ red recombinase[26]
 pKD4Kan, template plasmid[26]
 pSTV28Cm, expression using lac promotorTaKaRa
 pCP20Cm, Amp, yeast Flp recombinase gene, FLp[26]
 pET32aAmp, expresses a fusion fragment of the His tagThis study
 pGEX-4t-1Amp, expresses a fusion fragment of the GST tagThis study