Table 1 Bacterial strains and plasmids used in this study.

Strain

 DE205B

O2:K1, NalR

[21,22,23,24,25]

 DE205BΔireA

ireA Deletion mutant strain

[21]

 DE205BΔireA/ireA*

ireA Complemented strain

[21]

 DE205BΔ0007

0007 Deletion mutant strain

This study

 DE205BΔ0007/0007*

0007 Complemented strain

This study

 DE205BΔ0008

0008 Deletion mutant strain

This study

 DE205BΔ0008/0008*

0008 Complemented strain

This study

 DE205BΔ2235

2235 Deletion mutant strain

This study

 DE205BΔ2235/2235*

2235 Complemented strain

This study

 DE205BΔtonB

tonB deletion mutant strain

This study

 DE205BΔtonB/tonB*

tonB Complemented strain

This study

 DH5a

Competent invitrogen cells

Vazyme

 BL21

Competent invitrogen cells

Vazyme

Plasmid

 pKD46

Amp, expresses λ red recombinase

[26]

 pKD4

Kan, template plasmid

[26]

 pSTV28

Cm, expression using lac promotor

TaKaRa

 pCP20

Cm, Amp, yeast Flp recombinase gene, FLp

[26]

 pET32a

Amp, expresses a fusion fragment of the His tag

This study

 pGEX-4t-1

Amp, expresses a fusion fragment of the GST tag

This study