Strain or plasmid | Characteristics | References |
---|---|---|
Strain | ||
 DE205B | O2:K1, NalR | |
 DE205BΔireA | ireA Deletion mutant strain | [21] |
 DE205BΔireA/ireA* | ireA Complemented strain | [21] |
 DE205BΔ0007 | 0007 Deletion mutant strain | This study |
 DE205BΔ0007/0007* | 0007 Complemented strain | This study |
 DE205BΔ0008 | 0008 Deletion mutant strain | This study |
 DE205BΔ0008/0008* | 0008 Complemented strain | This study |
 DE205BΔ2235 | 2235 Deletion mutant strain | This study |
 DE205BΔ2235/2235* | 2235 Complemented strain | This study |
 DE205BΔtonB | tonB deletion mutant strain | This study |
 DE205BΔtonB/tonB* | tonB Complemented strain | This study |
 DH5a | Competent invitrogen cells | Vazyme |
 BL21 | Competent invitrogen cells | Vazyme |
Plasmid | ||
 pKD46 | Amp, expresses λ red recombinase | [26] |
 pKD4 | Kan, template plasmid | [26] |
 pSTV28 | Cm, expression using lac promotor | TaKaRa |
 pCP20 | Cm, Amp, yeast Flp recombinase gene, FLp | [26] |
 pET32a | Amp, expresses a fusion fragment of the His tag | This study |
 pGEX-4t-1 | Amp, expresses a fusion fragment of the GST tag | This study |